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Image Search Results
Journal: PLOS ONE
Article Title: An optimised protocol for the detection of lipofuscin, a versatile and quantifiable marker of cellular senescence
doi: 10.1371/journal.pone.0306275
Figure Lengend Snippet: Human dermal fibroblasts were treated with vehicle (PBS) or 20μM BMS-470539 for 6 days and the fluorescence signal determined at multiple channels before staining with SBB (A, denoting natural autofluorescence) and after staining with SBB (B, indicating positive detection of lipofuscin). Images were captured at 20X magnification using the EVOS FL Imaging System using the following Light Cubes: GFP (green, EX470/22—EM525/50), RFP (orange EX531/40—EM593/40), Texas Red (red, EX585/29—EM628/32), Cy5 (deep red, EX628/40—EM685/40), DAPI (blue, EX357/44—EX447/60) and transmitted channel. Scale bars represent 20μm.
Article Snippet: The cells were then washed twice in PBS for 10 minutes each, followed by a 10-minute incubation in a 1μg/ml
Techniques: Fluorescence, Staining, Imaging
Journal: PLOS ONE
Article Title: An optimised protocol for the detection of lipofuscin, a versatile and quantifiable marker of cellular senescence
doi: 10.1371/journal.pone.0306275
Figure Lengend Snippet: Human dermal fibroblasts were treated with vehicle (PBS) or BMS-470539 (1–20μM) for 6 days. Cells were assessed by histochemical determination of SA-β-Gal (A), detected by brightfield (BF) microscopy, and of lipofuscin by staining with Sudan Black B dye, which was detected by brightfield microscopy (B) and by fluorescence microscopy (Cy5 channel, co-stained with DAPI for nuclear detection, C). The percentage of cells showing positive staining for SA-β-Gal activity and lipofuscin were quantified for each staining technique. A minimum of 20 cells were analysed per technical replicate. Data represent the mean ± SEM (n = 4; One-way ANOVA with multiple comparisons correction vs. vehicle; ****p<0.0001). Representative images are shown in panel D. Brightfield images were captured using an EVOS XL Core Imaging System at 40X magnification and fluorescence images captured using an EVOS ™ FL Imaging System at 20X magnification. Scale bars represent 25μm.
Article Snippet: The cells were then washed twice in PBS for 10 minutes each, followed by a 10-minute incubation in a 1μg/ml
Techniques: Microscopy, Staining, Fluorescence, Activity Assay, Imaging