pbs solution with dapi Search Results


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ChemoMetec solution 8 ( dapi in pbs)
Solution 8 ( Dapi In Pbs), supplied by ChemoMetec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SERVA Electrophoresis dapi (4′-6-diamidino-2-phenylindole2hcl′)
Dapi (4′ 6 Diamidino 2 Phenylindole2hcl′), supplied by SERVA Electrophoresis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ChemoMetec solution 3 ( dapi, 0.1% triton x-100 in pbs)
Solution 3 ( Dapi, 0.1% Triton X 100 In Pbs), supplied by ChemoMetec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson dapi staining solution (1 μg/ml dapi in pbs)
Dapi Staining Solution (1 μg/Ml Dapi In Pbs), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ChemoMetec dapi pbs staining solution
Dapi Pbs Staining Solution, supplied by ChemoMetec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck & Co 1μg/ml dapi solution in pbs
Human dermal fibroblasts were treated with vehicle <t>(PBS)</t> or 20μM BMS-470539 for 6 days and the fluorescence signal determined at multiple channels before staining with SBB (A, denoting natural autofluorescence) and after staining with SBB (B, indicating positive detection of lipofuscin). Images were captured at 20X magnification using the EVOS FL Imaging System using the following Light Cubes: GFP (green, EX470/22—EM525/50), RFP (orange EX531/40—EM593/40), Texas Red (red, EX585/29—EM628/32), Cy5 (deep red, EX628/40—EM685/40), <t>DAPI</t> (blue, EX357/44—EX447/60) and transmitted channel. Scale bars represent 20μm.
1μg/Ml Dapi Solution In Pbs, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1μg/ml dapi solution in pbs/product/Merck & Co
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Dojindo Labs 1% bsa/pbs containing 1:1000 diluted dapi solution
Human dermal fibroblasts were treated with vehicle <t>(PBS)</t> or 20μM BMS-470539 for 6 days and the fluorescence signal determined at multiple channels before staining with SBB (A, denoting natural autofluorescence) and after staining with SBB (B, indicating positive detection of lipofuscin). Images were captured at 20X magnification using the EVOS FL Imaging System using the following Light Cubes: GFP (green, EX470/22—EM525/50), RFP (orange EX531/40—EM593/40), Texas Red (red, EX585/29—EM628/32), Cy5 (deep red, EX628/40—EM685/40), <t>DAPI</t> (blue, EX357/44—EX447/60) and transmitted channel. Scale bars represent 20μm.
1% Bsa/Pbs Containing 1:1000 Diluted Dapi Solution, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Dojindo Labs solution of 1 × 10− dapi (dojindo molecular technologies, japan) in pbs
Human dermal fibroblasts were treated with vehicle <t>(PBS)</t> or 20μM BMS-470539 for 6 days and the fluorescence signal determined at multiple channels before staining with SBB (A, denoting natural autofluorescence) and after staining with SBB (B, indicating positive detection of lipofuscin). Images were captured at 20X magnification using the EVOS FL Imaging System using the following Light Cubes: GFP (green, EX470/22—EM525/50), RFP (orange EX531/40—EM593/40), Texas Red (red, EX585/29—EM628/32), Cy5 (deep red, EX628/40—EM685/40), <t>DAPI</t> (blue, EX357/44—EX447/60) and transmitted channel. Scale bars represent 20μm.
Solution Of 1 × 10− Dapi (Dojindo Molecular Technologies, Japan) In Pbs, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/solution of 1 × 10− dapi (dojindo molecular technologies, japan) in pbs/product/Dojindo Labs
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Beyotime pbs solution containing 4',6-diamidino-2-phenylindole (dapi)
Human dermal fibroblasts were treated with vehicle <t>(PBS)</t> or 20μM BMS-470539 for 6 days and the fluorescence signal determined at multiple channels before staining with SBB (A, denoting natural autofluorescence) and after staining with SBB (B, indicating positive detection of lipofuscin). Images were captured at 20X magnification using the EVOS FL Imaging System using the following Light Cubes: GFP (green, EX470/22—EM525/50), RFP (orange EX531/40—EM593/40), Texas Red (red, EX585/29—EM628/32), Cy5 (deep red, EX628/40—EM685/40), <t>DAPI</t> (blue, EX357/44—EX447/60) and transmitted channel. Scale bars represent 20μm.
Pbs Solution Containing 4',6 Diamidino 2 Phenylindole (Dapi), supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbs solution containing 4',6-diamidino-2-phenylindole (dapi)/product/Beyotime
Average 90 stars, based on 1 article reviews
pbs solution containing 4',6-diamidino-2-phenylindole (dapi) - by Bioz Stars, 2026-03
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Image Search Results


Human dermal fibroblasts were treated with vehicle (PBS) or 20μM BMS-470539 for 6 days and the fluorescence signal determined at multiple channels before staining with SBB (A, denoting natural autofluorescence) and after staining with SBB (B, indicating positive detection of lipofuscin). Images were captured at 20X magnification using the EVOS FL Imaging System using the following Light Cubes: GFP (green, EX470/22—EM525/50), RFP (orange EX531/40—EM593/40), Texas Red (red, EX585/29—EM628/32), Cy5 (deep red, EX628/40—EM685/40), DAPI (blue, EX357/44—EX447/60) and transmitted channel. Scale bars represent 20μm.

Journal: PLOS ONE

Article Title: An optimised protocol for the detection of lipofuscin, a versatile and quantifiable marker of cellular senescence

doi: 10.1371/journal.pone.0306275

Figure Lengend Snippet: Human dermal fibroblasts were treated with vehicle (PBS) or 20μM BMS-470539 for 6 days and the fluorescence signal determined at multiple channels before staining with SBB (A, denoting natural autofluorescence) and after staining with SBB (B, indicating positive detection of lipofuscin). Images were captured at 20X magnification using the EVOS FL Imaging System using the following Light Cubes: GFP (green, EX470/22—EM525/50), RFP (orange EX531/40—EM593/40), Texas Red (red, EX585/29—EM628/32), Cy5 (deep red, EX628/40—EM685/40), DAPI (blue, EX357/44—EX447/60) and transmitted channel. Scale bars represent 20μm.

Article Snippet: The cells were then washed twice in PBS for 10 minutes each, followed by a 10-minute incubation in a 1μg/ml DAPI solution in PBS (Merck, D9542).

Techniques: Fluorescence, Staining, Imaging

Human dermal fibroblasts were treated with vehicle (PBS) or BMS-470539 (1–20μM) for 6 days. Cells were assessed by histochemical determination of SA-β-Gal (A), detected by brightfield (BF) microscopy, and of lipofuscin by staining with Sudan Black B dye, which was detected by brightfield microscopy (B) and by fluorescence microscopy (Cy5 channel, co-stained with DAPI for nuclear detection, C). The percentage of cells showing positive staining for SA-β-Gal activity and lipofuscin were quantified for each staining technique. A minimum of 20 cells were analysed per technical replicate. Data represent the mean ± SEM (n = 4; One-way ANOVA with multiple comparisons correction vs. vehicle; ****p<0.0001). Representative images are shown in panel D. Brightfield images were captured using an EVOS XL Core Imaging System at 40X magnification and fluorescence images captured using an EVOS ™ FL Imaging System at 20X magnification. Scale bars represent 25μm.

Journal: PLOS ONE

Article Title: An optimised protocol for the detection of lipofuscin, a versatile and quantifiable marker of cellular senescence

doi: 10.1371/journal.pone.0306275

Figure Lengend Snippet: Human dermal fibroblasts were treated with vehicle (PBS) or BMS-470539 (1–20μM) for 6 days. Cells were assessed by histochemical determination of SA-β-Gal (A), detected by brightfield (BF) microscopy, and of lipofuscin by staining with Sudan Black B dye, which was detected by brightfield microscopy (B) and by fluorescence microscopy (Cy5 channel, co-stained with DAPI for nuclear detection, C). The percentage of cells showing positive staining for SA-β-Gal activity and lipofuscin were quantified for each staining technique. A minimum of 20 cells were analysed per technical replicate. Data represent the mean ± SEM (n = 4; One-way ANOVA with multiple comparisons correction vs. vehicle; ****p<0.0001). Representative images are shown in panel D. Brightfield images were captured using an EVOS XL Core Imaging System at 40X magnification and fluorescence images captured using an EVOS ™ FL Imaging System at 20X magnification. Scale bars represent 25μm.

Article Snippet: The cells were then washed twice in PBS for 10 minutes each, followed by a 10-minute incubation in a 1μg/ml DAPI solution in PBS (Merck, D9542).

Techniques: Microscopy, Staining, Fluorescence, Activity Assay, Imaging